Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results
نویسندگان
چکیده
منابع مشابه
Recommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop.
The in vivo alkaline single cell gel electrophoresis assay, hereafter the Comet assay, can be used to investigate the genotoxicity of industrial chemicals, biocides, agrochemicals and pharmaceuticals. The major advantages of this assay include the relative ease of application to any tissue of interest, the detection of multiple classes of DNA damage and the generation of data at the level of th...
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Although the rodent comet assay is gaining acceptance as a standard technique for evaluating DNA damage in vivo, there is no internationally accepted guideline for its conduct and several aspects of its experimental design have not been optimized. For example, no standard positive control is used, there is no agreement on how tissue toxicity should be measured and sources of experimental variab...
متن کاملRecommendations for increasing alkaline comet assay reliability in plants.
In plants, an increasing interest for the comet assay was shown in the last decade. This versatile technique appears to be promising to detect the genotoxic effect of pollutants and to monitor the environment. However, the lack of a standardised protocol and the low throughput of the assay limit its use in plants. The aims of this paper are to identify key factors affecting comet assay performa...
متن کاملStatistical analysis of comet assay results
The comet assay can distinguish small differences in DNA damage between different samples of cells, implying that statistical tests are important to assess whether this occurs by chance. Excellent scholarly papers with concise descriptions of statistical analysis and recommendations for tests have been published (Lovell et al., 1999; Lovell and Omori, 2008). We often come across publications th...
متن کاملStochastic modeling for the COMET-assay
We present a stochastic model for single cell gel electrophoresis (COMET-assay) data. The distribution of length of DNA fragments is calculated according to a ’Random Breakage Model’ and the migration of DNA fragments among gel fibers is discussed. Essential to our approach is the use of point process structures, renewal theory and reduction to intensity histograms for further data analysis. Pa...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2020
ISSN: 1754-2189,1750-2799
DOI: 10.1038/s41596-020-0398-1